Molecular Diversification and Frequency of mecA Gene in the MRSA Infections of Hospitalized Patients at Islamabad, Pakistan

Objective: To determine the molecular diversification and frequency of mecA Gene in MRSA infections hospitalised patients at Islamabad, Pakistan.
 Study design: Cross-sectional study.
 Place Duration: Pakistan Institute Medical Science, Pakistan, from Sep 2017 to 2018.
 Methodology: explore occurrence pathogenic S. aureus 500 samples were collected. All isolates further characterised by gram staining, catalase test DNAse media analysis validating presence S.aureus. The overall S.aureus was 19.5% all isolates. Six highly distinguished colonies evaluated through colony PCR 16S r RNA analysis, results revealed 99.9% homology with degree resistance for 12 based on previous pattern. extraction concentration DNA done CTAB Nanodrop methods. Then primers designed gene aureus.
 Results: Results showed 51 (52.7%) MSSA 45 (47.4%) among Colony 97 (99.9%) twelve extracted DNAs isolates.
 Conclusion: study is first report Islamabad. It has depicted a resistant gene's rising trend aureus.